Troubleshooting Diagnostic IHC Testing

Recorded On: 10/15/2020

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Studies have shown that up to 30% of all immunohistochemical tests are sub-optimal for interpretation which can impact patient diagnosis and, subsequently, treatment. This lecture will highlight common problems that can occur during pre-analytical, analytic, and post-analytic stages of testing. Specific topics including fixation, microtomy, antigen-retrieval, primary antibody selection, and use of appropriate controls will be discussed.

CEUs: This histology course is worth 1 continuing education credit. Course is available for 365 days from date of purchase. 

Richard Cartun, MS, PhD

Director, Histology/Morphologic Proteomics

Hartford Hospital

I currently serve as the Director of Histology, Immunopathology, and the Martin M. Berman, MD Morphologic Proteomics Laboratory at Hartford Hospital, a large tertiary care hospital in Hartford, CT.  I have been doing immunohistochemical testing for more than 40 years now and, as a result, have extensive knowledge in the technical aspects and interpretation of this valuable laboratory test.


Troubleshooting Diagnostic IHC Testing
Open to view video.
Open to view video. Objectives: Learn the importance of pre-analytical factors on successful IHC testing. Learn how antigen retrieval can cause false negative results. Learn when to run a negative reagent control.
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2 Questions
2 Questions Please complete the survey to receive your CEU certificate.
1.00 CEUs credit  |  Certificate available
1.00 CEUs credit  |  Certificate available