Theory and Application of Silver Stains in the Histology Laboratory

Recorded On: 06/24/2020

Registration Closed

The silver stains used and performed in the histology lab are complex and diverse. They provide an everyday unique challenge to histologic preparation and staining results. Generally, five various chemical categories are used for this application. These include argentaffin, argyrophilic, impregnation, oxidation-reduction, and metallic-metallic reactions. They stain and demonstrate a wide array of tissue components and constituents including bacteria, fibers, basement membranes, carbohydrates, glycoproteins, and axons. Examples and chemical mechanisms of each technique will be examined and discussed. Other nuances of these procedures will be discussed from an end user perspective. Stain automation has prevented a full comprehension of key reactions that occur during these silver procedures.

CEUs: This histology course is worth 1 continuing education credit. Course is available for 365 days from date of purchase.

Debra Siena, HT(ASCP),QIHC

Technical Manager

Statlab Medical Products

Debbie Siena has served as Technical Support Manager for StatLab Medical Products since September, 2010. Debbie has over 25 years of experience in IHC, routine histology and currently focuses on troubleshooting product issues in partnership with customers and suppliers, educating sales and customer service team members on IHC, histology, and overseeing our Customer Outreach Program. Prior to StatLab, Debbie was with Ventana Medical Systems, Inc. for seven years as a Technical Marketing Representative, Senior Technical Marketing Specialist, Area Support Manager and Key Account Technical Specialist with the Corporate and Major Account Team. While at Ventana, Debbie was a multiple award winner, including Technical Specialist of the Year, Technical Specialist of the Quarter, and President’s Club member. Prior to Ventana, Debbie was the Manager of the Anatomic Pathology Department at Baylor University Medical Center in Dallas, Texas for seven years and served as Supervisor of Histology at Children’s Medical Center in Dallas, Texas. While at Children’s Medical Center, Debbie was responsible for development of the IHC and ISH procedures and techniques. Debbie is very active in continuing education and has presented numerous educational workshops at the state and national level as well as multiple teleconferences for NSH.