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The Total Test Approach to IHC Standardization

Recorded On: 07/22/2020

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Standardization is a continual challenge in immunohistochemical testing. When considering the various parameters involved (i.e. tissue acquisition, fixation, protocol steps, reagents, instrumentation), this is not surprising; however, consistency and reproducibility are more important than ever. The prominence of personalized medicine, particularly companion diagnostics, has increased clinical accessibility to antibodies capable of identifying mutated or abnormally expressed proteins which are then targeted by therapeutic drugs. Consistency and reproducibility are also important in research in order to validate antibodies and their results. Aside from understanding the significance, the process and approach to standardization can be overwhelming. In the 1990s, a more rigorous ‘Total Test’ approach was recommended for IHC assays. This approach offers a comprehensive, methodical review by categorizing the factors that affect consistency and reproducibility. This session will explore the challenges to standardization factored into the approach, and how it can be utilized to improve standardization in any laboratory setting.

Kimberly Feaster, HTL(ASCP)QIHC

Program Director, Histology

Kimberly Feaster is a professor at West Virginia University.  Ms. Feaster has experience as a bench Histotechnologist and supervisor in research and hospital histology laboratories. She is currently the director of the Histotechnology program at West Virginia University. She holds a masters degree, her HTL, and her QIHC.  In addition to running the program at West Virginia – she is an active volunteer with the NSH.  She is an active member of the NSH Education Committee.  Volunteering her time and expertise for many years to the various projects and programs

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The Total Test Approach to IHC Standardization
Open to view video.
Open to view video. Objectives: Recognize the significance and challenges to standardization; Identify influential factors within each phase of the process; Formulate a plan to implement the total test approach or improve upon your existing process.
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2 Questions
2 Questions Please complete the survey to receive your CEU certificate.
Certificate
1.00 CEUs credit  |  Certificate available
1.00 CEUs credit  |  Certificate available