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QIHC Prep Course

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The NSH QIHC Prep Course is intended to provide participants with an overview of the principles and practices of immunohistochemistry in an effort to comprehensively prepare them for the ASCP’s QIHC examination. The course covers the topic areas outlined by the ASCP’s published exam topic outline, and offers suggestions for not only trying to meet the minimum requirements, but also become knowledgeable enough to be truly ‘qualified’.

Every effort is made to address all of the technical aspects of the aforementioned procedures, including coverage of the underlying science of IHC, heat- and digestion-induced ‘retrieval techniques, various ‘quenching and ‘blocking’ procedures, commonly employed detection methods, regulatory requirements for procedure validation, quality control, troubleshooting, and use of automated staining systems.

Learning Section Format: The course contains a three hour lecture broken into 8 "digestible" sections.
 
Additional Materials: Included with this course is an indexed Resource Guide with seventy plus pages of study material and PDF handouts for each section.
 
Knowledge Check Question Bank: At the conclusion of Section 8 you will have access to a question bank to test your knowledge and practice for the exam. 

CEUs: This histology course is worth 5 continuing education credit. Course is available for 365 days from date of purchase.

Joe Myers, MS, CT(ASCP)QIHC

Joe Myers has earned and renewed his QIHC credential so he truly knows what it takes. He is currently employed as a Senior Technical Sales Specialist with Biocare Medical, LLC, a vendor of IHC/ISH reagents and instruments. He has extensive experience with immunohistochemistry (IHC), in situ hybridization (ISH), and heat-induced epitope retrieval (HIER) procedures, and has written a variety of papers in peer-reviewed journals on the application of IHC/ISH in the diagnosis of cancer and infectious disease and in laboratory ‘trade’ publications on automated IHC slide staining systems, HIER techniques, multi-antigen immuno-staining procedures and devices, and cost-reduction methods. Joe began his career at, and 'founded' the Immunocytochemistry Lab at the Johns Hopkins Medical Institutions in 1984. Before entering the technical-sales arena, Joe was employed as the Administrative Director of Clinical Lab Services in two hospitals. From 2012 to 2014, Joe served as chairperson of the National Society for Histotechnology’s IHC Resource Group.

Key:

Complete
Failed
Available
Locked
Resource Guide
Resource Guide
Open to download resource.
Open to download resource. Instructor, Joe Myers, has created a resource guide for continued study of the topics he includes in the following lecture. During the lecture the guide is referenced by Mr. Myers as the "Handout". The PDFs listed in the Handout Tab are powerpoint slides for each section only.
Lectures
Section 1: Introduction & Course Overview
Open to view video.
Open to view video. In Section 1 presenter Joe Myers provides an overview of the exam and the information available on the ASCP website.
Section 2: How Does Immunostaining Work?
Open to view video.
Open to view video. Section 2 provides an overview of the essential procedural ‘steps’ with special emphasis on pretreatments and ‘primary’ antibodies.
Section 3: Detection Methods
Open to view video.
Open to view video. Provides a short history of the development of immuno-staining procedures, describes the underlying science of commonly-employed techniques, and outlines other important issues such as species-specificity and enzyme (label) and chromogen compatibility.
Section 4: Clinical/Diagnostic Application of IHC
Open to view video.
Open to view video. Provides a very cursory overview of some common clinical/diagnostic (and research-oriented) applications of IHC procedures, demonstrated through a variety of photomicrographs.
Section 5: Procedure Optimization & Validation
Open to view video.
Open to view video. Provides a comprehensive overview of regulatory/accreditation-agency standards relating to IHC, and should stimulate some thought on the part of program participants about the differences in validating ‘quantitative’ (objective?) and ‘qualitative’ (subjective?) laboratory procedures.
Section 6: Troubleshooting
Open to view video.
Open to view video. Provides an comprehensive overview of the steps that should be taken by manufacturers of IHC reagents and the labs in which these products are employed when appropriate/expected staining results are not obtained, and offers suggestions for corrective actions in the form of tables and flow diagrams.
Section 7: Commonly Employed Instruments
Open to view video.
Open to view video. Provides a cursory review of the differences in designed and operation of IHC slide-staining systems and is should stimulate in participants what the presenter calls a ‘healthy distrust’ of such instruments.
Section 8: Recommended Reading & Course Conclusion
Open to view video.
Open to view video. In the final section Mr. Myers discussed recommended reading resources to study for the exam and concludes the instruction portion of the course.
Participant Survey
6 Questions
6 Questions Please complete the survey to receive your CEU certificate.
Certificate
5.00 credits  |  Certificate available
5.00 credits  |  Certificate available
Question Bank
Basic Level Questions
46 Questions  |  Unlimited attempts  |  0/46 points to pass
46 Questions  |  Unlimited attempts  |  0/46 points to pass The following questions were created to help you better prepare for the examination. These questions are not solely derived from the lectures you just completed, but from subject matter experts and other IHC resources created by the NSH. These are basic level questions.
Intermediate Level Questions
46 Questions  |  Unlimited attempts  |  0/46 points to pass
46 Questions  |  Unlimited attempts  |  0/46 points to pass The following questions were created to help you better prepare for the examination. These questions are not solely derived from the lectures you just completed, but from subject matter experts and other IHC resources created by the NSH. These are intermediate level questions.
Advanced Questions
32 Questions  |  Unlimited attempts  |  0/32 points to pass
32 Questions  |  Unlimited attempts  |  0/32 points to pass The following questions were created to help you better prepare for the examination. These questions are not solely derived from the lectures you just completed, but from subject matter experts and other IHC resources created by the NSH. These are advanced level questions.