Morphological Artifact Free Solutions for Ocular Histology

The Journal of Histotechnology's passion for advancing ocular histology can be dated back to its birth year, 1977[1]. In addition to embedding medium and microtomy, many technical improvements have been made in the last half century. In its 45th anniversary special issue this December, the Journal will feature manuscripts, including Dr. Wang's, that address scientific and technical advancements in ocular histology field.  

This general session will provide insight if you struggle to achieve mouse eyeball preparation free of distortion such as shrinking and eyeball wall denting during fixation and paraffin processing, or if you have difficulty keeping ocular sections on glass slides while performing immunohistochemistry (IHC) or in situ hybridization (ISH) assays. In addition to reviewing the modified Davidson’s fixative and window technique for eyeball specimens, I will introduce our simplified and highly effective procedure for performing ocular histology which includes a novel fixative and a modified glass coating method. 

[1] Fischer MW. Embedding Paraffins for Eye Microtomy. J Histotechnol. 1977; 1(2); 67.  DOI: 10.1179/his.1977.1.2.67