Basic IHC: Understanding The Process To Improve Your Results
Recorded On: 10/15/2020
Immunohistochemical staining is now so commonly performed by instruments or robots that the actual staining process is lost in the barcodes and software, bottles and vials. This workshop dives back down to the basics of what is really happening on the slide: from tissue preparation to the sequential application of primary antibody, linking antibodies, enzyme complex, chromogen and counterstain. We will discuss the effects that pre-analytical events such as fixation, processing and microtomy have on the staining process as well as the results. Multiple staining methods will be examined including avidin-biotin, polymer and other biotin-free systems, as well as newer amplification techniques. And as we examine the process we will discuss how each step leads to the success or failure of the final product. A strong foundation of such basic understanding leads to ease in troubleshooting any unexpected staining results.
CEUs: This histology course is worth 1 continuing education credit. Course is available for 365 days from date of purchase.
Beth Roche, HTL(ASCP)
Until her retirement in April 2019, Beth Roche, HTL (ASCP) was a senior technical consultant at Roche Diagnostics with 40 years’ experience in the field of histotechnology. Experience in clinical and research laboratories with the University of Florida, Baxter Healthcare, WL Gore & Associates, and Ventana Medical Systems/ Roche Diagnostics gives her a hands-on background in the field. Additionally, she has trained technologists from labs across the world. Blending a background in basic histotechnology with cutting edge technology and equipment and more recent applications in immunohistochemistry, she brings both breadth and depth of experience to her seminars and presentations. A frequent presenter at national and state meetings, she enjoys the opportunity to discuss a wide variety of applications and situations with her audiences.
Her strength is in breaking down topics into simple, easy-to-follow steps; in encouraging the exchange of information among attendees; and in seeing when her students and attendees reach that “Aha!” moment of understanding.