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Education & Training Resources to help you upskill your knowledge and technical expertise in the lab.

  • Managing Toxic and Disruptive Behavior
    In this webinar we will explore the toxic behaviors we find in the workplace. We will not only identify the disruptor, but those around them that also add to the situation. We will discuss the impact on the individuals, the team and the organization, and learn of some techniques to minimize this. We will close with strategies to alleviate the symptoms of being a “Toxic Handler”.
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  • 2020 Poster Podcast Interviews: P03- Anatomy and mechanical properties of the fallopian tubes
    Falloposcopy is the endoscopic examination of the fallopian tubes. The fallopian tubes are challenging to access due to their deep location, small opening from the uterus, and lumen filled with epithelial-covered plicae. We and others have developed endoscopes for the evaluation of the ovaries and fallopian tubes, which are inserted through the uterus and tubal ostium. In order to understand how best to utilize these endoscopes, either as standalone devices or in concert with everting delivery balloons, we performed a preliminary study into the anatomy and mechanical behavior of the fallopian tubes using ex vivo porcine and human tissue. We investigated the mechanical properties through burst pressure, compliance testing, and balloon introduction at three locations: the isthmus, ampulla and infundibulum. We found that porcine fallopian tubes can tolerate a pressurization with saline of 15 psi for 1 minute with no histologically-apparent damage. Inflation of a balloon to the same pressure causes no rupture or apparent damage to the muscular layer of the fallopian tube, but movement of the balloon within the fallopian tube can denude the epithelial layer. Burst pressure testing on both human and porcine fallopian tube tissue showed that human tissues had on average higher burst pressures values than porcine tissue. These studies suggest that porcine tissue is a reasonable model for damage caused human tissue by falloposcopes.
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  • 2020 Poster Podcast Interviews: P08- Utilizing Image Analysis to Evaluate Overuse of Hematoxylin and Eosin Staining Reagents from Multiple Manufacturers
    There are no defined guidelines or industry standards as to how often Hematoxylin and Eosin (H&E) staining reagents need to be changed to maintain staining quality. In a previous study Premier Laboratory investigated the effects of overusing one set of H&E reagents on H&E staining quality ((2018) Short Poster Abstracts, Journal of Histotechnology, 41:4, 182-194, DOI:10.1080/01478885.2018.1523902). The results of that study show that the optical density of Hematoxylin stained regions remained consistent, while the optical density of Eosin stained regions decreased slightly as increasing numbers of slides were stained. This current study was designed as a follow up study in order to assess how H&E reagents made by 4 different manufacturers are impacted by overuse. This study utilized a custom image analysis algorithm that can calculate the optical density (OD) of both the nuclear and cytoplasmic components of H&E staining reagents to determine when overuse of reagents impacts staining quality. A variety of formalin fixed, routinely processed, paraffin embedded (FFPE) tissues were selected to represent a range of anticipated staining intensities. Four (4) identical multi-tissue blocks were prepared. Serial sections from each of the blocks were cut at 4µm. One section from the multi-tissue block was placed within each rack of 20 slides stained, for a total of 150 multi-tissue slides spaced over 3000 slides. Fresh H&E staining solutions were used and over the course of one week 3000 slides were stained with each manufacturers reagents. Slides were scanned and then analyzed via image analysis to determine any changes in optical density of the nuclear and cytoplasmic staining components.
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  • 2020 Poster Podcast Interviews: P09- A Modified Procedure For The Preparation of Rodent Heart Sections Using HistoGel Infusion
    The purpose of this work is to describe a procedure for the processing of rodent hearts that consistently provides microscopic visualization in situ of all four cardiac valves simultaneously in three histologic sections while preserving the integrity of the valves and their spatial relationship to other cardiac structures. Typically for routine toxicological studies, a single mid-sagittal section of the heart is created; however, the cardiac valves are not prioritized in those types of sections, and the presence and orientation of the valves tend to be haphazard. We propose an alternate method that utilizes a product called HistoGel.
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  • 2020 Poster Podcast Interviews: P10- High-resolution histological preparation of Araneomorphae and Mygalomorphae chelicerae using a modified petrographic technique
    Abstract: Producing quality histological preparations of spider chelicerae with articulated fangs and cheliceral teeth is exceptionally challenging, if not impossible, using conventional histology techniques. Typically, these structures are examined with topographic or radiographic imaging methods, such as scanning electron microcopy (SEM) and micro computed tomography (mIcro-CT). While both are very useful tools for morphological analysis, they’re not capable of revealing the fine tissue structure and cellular details, that a histological section viewed under light microscopy can provide. This study describes a modified petrographic/hard tissue histology technique to prepare high-resolution histology sections, for qualitative and quantitative assessment of both cheliceral soft tissue and fang microstructure.
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